Effects of Incubator Temperature and Oxygen Concentration During the Plateau Stage of Oxygen Consumption on Turkey Embryo Long Bone Development

Abstract

Temperature (TEM) and O2 concentrations during the plateau stage of oxygen consumption are known to affect yolk utilization, tissue development, and thyroid metabolism in turkey embryos. Three experiments were conducted to evaluate these incubation effects on long bone development. Fertile eggs of Nicholas turkeys were used. In each trial, standard incubation conditions were used to 24 d, when the eggs containing viable embryos were randomly divided into 4 groups. Four experimental cabinets provided 4 TEM (36, 37, 38, or 39°C) or 4 O2 concentrations (17, 19, 21, or 23% O2). In the third experiment, 2 temperatures (36 and 39°C) and 2 O2 concentrations (17 and 23%) were evaluated in a 2 × 2 factorial design. Body and residual yolk weights were obtained. Both legs were dissected, and shanks, femur, and tibia weights, length, and thickness were recorded. Relative asymmetry of each leg section was calculated. Chondrocyte density was evaluated in slides stained with hematoxylin and eosin. Immunofluorescence was used to evaluate the presence of collagen type X and transforming growth factor β. Hot TEM caused reduction of tibia weights and increase of shank weight when compared with cool TEM. The lengths of femur, tibia, and shanks were reduced by 39°C. The relative asymmetry of leg weights were increased at 38 and 39°C. Poult body and part weights were not affected by O2 concentrations, but poults on 23% O2 had bigger shanks and heavier tibias than the ones on 17% O2. High TEM depressed the fluorescence of collagen type X and transforming growth factor β. The O2 concentrations did not consistently affect the immunofluorescence of these proteins. The chondrocyte density was affected by TEM and O2 in resting and hypertrophic zones. In the third experiment, high TEM depressed BW, leg muscle weights, and shank length. Low O2 reduced tibia and shanks as a proportion of the whole body. We concluded that incubation conditions affect long bone development in turkeys.