Effects of inactivated Bordetella pertussis on phosphodiesterase in the lung of ovalbumin sensitized and challenged rats.

Ya-Juan Wang,Ji-Qiang Chen,Qiang-Min Xie,Xue-Feng Wang,Shun-De Song,Jun-Chun Chen,Ya-Li Jiang,Hui-Fang Tang,Zi-Gang Li

doi:10.1155/2014/581738

Abstract

This paper indicated that inactivated Bordetella pertussis (iBp) can enhance the lung airway hyperreactivity of the rats sensitized and challenged with OVA. The mechanisms were involved in the upregulation of cAMP-PDE activity and PDE4A, PDE4D, and PDE3 gene expression in the lungs. But only PDE4 activity was different between the OVA and OVA+iBp groups, and PDE4D expression was significantly increased in iBp rats alone. So, our data suggested that cosensitization with OVA and iBp affects lung airway reactivity by modulating the lung cAMP-PDE activity and PDE4D gene expression.

Highlights

Inactivated Bordetella pertussis has been used as a strong Th2 adjuvant to boost allergic responses to antigen such as house dust mite antigen (HDM), ovalbumin (OVA), and ragweed pollen in animal models of asthmatic hypersensitivity from 1968 [1,2,3,4]
Inactivated B. pertussis has long been used as an effective adjuvant for eliciting IgE responses to a variety of antigens in experimental animals, while being an antigen [16,17,18,19]
Because of this specific property, Inactivated Bordetella pertussis (iBp) has been used in animal models of hypersensitivity to boost allergic responses to HDM, OVA, and ragweed pollen

Summary

Introduction

Inactivated Bordetella pertussis (iBp) has been used as a strong Th2 adjuvant to boost allergic responses to antigen such as house dust mite antigen (HDM), ovalbumin (OVA), and ragweed pollen in animal models of asthmatic hypersensitivity from 1968 [1,2,3,4]. Our experiments have suggested that simultaneous exposure to OVA and intramuscularly iBp can enhance the bronchial hyperresponsiveness [6]. How this occurs at the molecular level has not been elucidated. The phosphodiesterase (PDE) superfamily participates in the only cellular pathways for degradation of the ubiquitous intracellular second messengers It comprises eleven biochemically and pharmacologically distinct enzyme families (PDEs 1-11) that hydrolyze cAMP and/or cGMP [7]. PDE4 is specific for cAMP and comprises four subtypes (A, B, C, and D) It is predominantly expressed and plays an important role in the regulation of cellular functions in inflammatory and immune cells. Whether the adjuvants had effects on PDE activity and expression was unclear

Methods

Results

Conclusion