Abstract
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.
Highlights
Osteoprotegerin (OPG) and its ligand, receptor activator of nuclear factor-kB ligand (RANKL), are critical factors in regulating the differentiation and maturation of osteoclasts, as well as bone resorption [1]
Human periodontal ligament fibroblasts (HPDLFs) are the primary cell type in the periodontal ligament and they contribute to the integrity of the periodontium
The aim of this study was to explore the influence of IL-10 and elevated glucose concentrations on the expression of OPG and RANKL in HPDLFs
Summary
Osteoprotegerin (OPG) and its ligand, receptor activator of nuclear factor-kB ligand (RANKL), are critical factors in regulating the differentiation and maturation of osteoclasts, as well as bone resorption [1]. The equilibrium between OPG and RANKL activity has an essential role in the homeostasis of bone metabolism. In the pathological process of periodontal disease, the OPG/RANKL equilibrium is disrupted, leading to increased bone resorption [2,3]. HPDLFs express both OPG and RANKL, affecting the formation of osteoclasts by modulating the OPG/RANKL equilibrium [4]. Multiple cytokines, which have different effects on the expression of OPG and RANKL, are involved in the pathogenesis of periodontal disease [5]. The regulatory effect of IL-10 on the expression of OPG and RANKL has not yet been defined
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