Abstract
To evaluate angiogenesis and reepithelization of colonic anastomoses in rats. 82 Wistar rats, divided into: young normoglycemic, old normoglycemic and hyperglycemic. Diabetes was induced with streptozotocin. Glycemia was assessed before induction, at 24 hours and after 90 days, when a colotomy and an anastomosis were performed, assessed at days 3, 7, and 14. Samples were prepared by immuno-histochemistry (PCNA and antifactor VIII). Mean glycemia after 90 days streptozotocin induction was 244,95 mg/dl. Day 7, reepithelization was greater in the young group than in the old normoglycemic (p<0.0001) and old hyperglycemic (p<0.0001) groups. Day 14, the differences were significant between the young and old normoglycemic (p<0.0001) and old hyperglycemic (p<0.0001) groups. The two old groups were not significantly different. At the three periods angiogenesis was higher in the young group than in the old normoglycemic (p3=0.014; p7<0.0001; p14<0.0001) or the old hyperglycemic groups (p3=0.014, p7<0.0001; p14<0.0001). The old groups, day 3, were not different (p3=0.627), but days 7 and 14, angiogenesis was bigger in the normoglycemic group (p7=0.042; p14=0.005). Age is important in reepithelization and angiogenesis of colonic anastomoses. Hyperglycemia interferes mainly in angiogenesis.
Highlights
Wound healing is a very complex process and, for this reason, it can be negatively impacted by several factors, including advanced age and diabetes, and often by the combination of both
As for the gastrointestinal tract, studies show that anastomosis strength is preserved, while collagen deposition, migration of inflammatory cells, and reepithelization are changed . [6,7,8] This study aims to analyse reepithelization and angiogenesis of colonic anastomoses in young and old normoglycemic rats comparing them to old chronically hyperglycemic rats
At day 7, the mean number of vessels was 25.49% higher in the young normoglycemic group (11.77 mean) in comparison with the old normoglycemic group (8.77 mean; p
Summary
Wound healing is a very complex process and, for this reason, it can be negatively impacted by several factors, including advanced age and diabetes, and often by the combination of both. Through the nonenzymatic glycosylation mechanism, causes lesions in almost all kinds of tissues and can have a significant impact on the development of the healing process in colonic anastomoses by impairing its strength, the migration of inflammatory cells, delaying reepithelization and reducing the quality of collagen deposition and new vessel formation . [1,2,3,4,5] Old age has been described as a hindering factor in the healing process. As for the gastrointestinal tract, studies show that anastomosis strength is preserved, while collagen deposition, migration of inflammatory cells, and reepithelization are changed . As for the gastrointestinal tract, studies show that anastomosis strength is preserved, while collagen deposition, migration of inflammatory cells, and reepithelization are changed . [6,7,8] This study aims to analyse reepithelization and angiogenesis of colonic anastomoses in young and old normoglycemic rats comparing them to old chronically hyperglycemic rats
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