Effects of hyperbaric oxygen therapy in improvement of Tnf- Α, Hsp 70, Enos, Vegf, collagen 3, and Il 6 protein expression in wound healing

Abstract

Wound healing is a progressive physiological process with overlapping stages. Hyperbaric oxygen therapy increases tissue oxygenation, thereby increasing tissue oxygenation and the formation of H2O2 as the second messenger of the phosphorylation of tumor necrosis factor α, NOS, VEGF, and nuclear factor β-kappa. This further study aims to determine whether hyperbaric oxygen therapy can increase collagen 3, HSP 70, IL6 expression and wound healing. This study is an animal study using "pre-test and post-test design of a randomized control group". Divided into 4 groups (HBO 2.4 ATA 3x30 minutes, 10 sessions HBO 2.4 ATA 3x30 minutes, control group without HBO), each group consisted of 7 groups with 28 male rats. Excision of the wound 1x1cm was performed. The distribution of the data was analyzed by SPSS. Hyperbaric oxygen therapy increased the expression of Collagen 3 (p=0.04), HSP 70 (p=0.03), IL 6 (p=0.02) and wound healing (p=0.002) with 5 sessions of HBO 2.4 ATA and 10 sessions of HBO 2.4 ATA increased Collage 3 (p=0.02), HSP 70 (p=0.04), L6 expression significantly (p=0.02) but did not significantly improve wound healing (p=0, 3) compared without HBO.