Abstract
Objective To explore the effects of HBO preconditioning on the inflammatory reaction induced by ischemia-reperfusion following skin flap transplantation in rats.Methods Fifty-six Sprague-Dawley rats were randomly divided into 7 different groups:the sham group,the ischemia-reperfusion(IR)day 1 group,the IR day 3 group,the IR day 5 group,the HBO+ischemia-reperfusion day 1 group,the HBO+ischemiareperfusion day 3 group and the HBO+ischemia-reperfusion day 5 group.First,the abdominal pedicle skin flap model was developed and the animals were treated with HBO preconditioning.Then,levels of MMP-9 and HMGB1 were measured with ELLSA.Results The MMP-9 levels of IR d 1 and d 3 groups,the HMGB1 levels of d 1,3,5 groups were significantly higher than those of the sham group[(13.74±2.04)、(11.05±1.35)μg/L](P＜0.01).The MMP-9 level of IR d 5 group[(16.66±2.45)μg/L]was higher than that of the sham group(P＜0.05).The MMP-9 levels of the HBO+ischemia-reperfusion d 1 and d 3 groups were lower than that of the IR group on the same day(P＜0.05).The HMGB1 level of the HBO+ischemia-reperfusion d 5 group was obviously lower than that of the IR group on the same day(P＜0.01).Conclusions Hyperbaric oxygen preconditioning might alleviate inflammatory reaction induced by ischemia-reperfusion through the access of decreasing the serum levels of MMP-9 and HMGB1 in rats following skin flap transplantation. Key words: Hyperbaric oxygen; Skin flap; MMP-9; HMGB1
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