Effects of hyperbaric oxygen on the level of interleukin-1β and interleukin-10 in the lung tissue of mice following cerebral ischemia-reperfusion

Abstract

Objective To observe the effects of hyperbaric oxygen (HBO) on the level of IL-1β and IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,and also to investigate their role and significance.Methods Adult male BALB/c mice were randomly divided into the ischemia-reperfusion group (or the IR group),the HBO group and the sham group.Cerebral ischemia-reperfusion model was established by clamping bilateral carotid arteries of mice.Level of IL-1β and IL-10 in the lung tissue at h 4,24 and 48 were measured respectively after cerebral ischemia-reperfusion by ELISA.Results The levels of IL-1β in the lung tissue of mice in the hyperbaric oxygen group and the ischemia-reperfusion group at h 4 were significantly higher than that of the sham group at the same time point (P ＜0.01).No statistical significance in the IL-1β levels could be seen in the lung tissue of mice between the HBO group and the ischemia-reperfusion group.The levels of IL-1β at h 24 for the HBO group and the ischemia-reperfusion group were all significantly higher than that of the sham group at the same time point (P ＜0.05,P ＜0.01).The level of IL-1β in the lung tissue of mice for the HBO group was significantly lower than that of the IR group at the same time point (P ＜ 0.05).The level of IL-10 in the lung tissue of the HBO group was significantly higher than those of the IR group and the sham group (P ＜ 0.05).Conclusions HBO could decrease the level of IL-1β and increase the level of IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,then it could offer some protection for acute lung injury induced by cerebral ischemia-reperfusion. Key words: Hyperbaric oxygen; Cerebral ischemia reperfusion; Acute lung injury; Inflammatory cytokine ; Mice