Effects of hyperbaric oxygen on survival rate of ultralong random-pattern flaps in rats

Abstract

Objective To investigate effects of hyperbaric oxygen on survival rate of ultralong randompattern flaps in rats. Methods Sixty male Wistar rats were randomly divided into 3 groups. A random-pattern flap was elevated in the central part of the rachis of each rat in 3 groups. After operation, the rats in control group were left as controls; those in normal pressure oxygen group were began to receive normal pressure oxygen twice a day lasting for five days and one time went on one hour; those in hyperbaric oxygen group were exposed to hyperbaric oxygen twice a day lasting for five days and one time went on one hour. On the seventh day, all experiments rats were put to death and survival rates of all flaps were calculated. And the expression levels of vascular endothelial growth factor (VEGF) and CDI05 were measured. Results On the seventh day, the survival rates in 3 groups were as follows: control group, (48.6～8.0)% ; normal pressure oxygen group,(51.7±7.1)%; hyperbaric oxygen group, (89.1±3.7)%. The differences were statistically significant among the 3 groups. Expression levels of VEGF and CDI05 were significant higher in hyperbaric oxygen group than in the other groups. And there was no significant difference between normal pressure oxygen group andcontrol group. Conclusions Hyperbaric oxygen can significantly increase flap survival rate of random-pattern flap in rats, while normal pressure oxygen doesn't have a certain effect under the same condition. Key words: Hyperbaric oxygen; Normal pressure oxygen; Random-pattern flap; Vascular endothelial growth factor; CD105