Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage.

Lixia Wei,Jiwen Wang,Qing Ren,Yongjun Zhang

doi:10.1590/s0102-865020170040000002

Lixia Wei, Jiwen Wang + Show 2 more

Open Access

https://doi.org/10.1590/s0102-865020170040000002

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Abstract

To evaluate the effects of HBO (Hyperbaric oxygen) and NGF (Nerve growth factor) on the long-term neural behavior of neonatal rats with HIBD (Neonatal hypoxic ischemic brain damage). The HIBD model was produced by ligating the right common carotid artery of 7 days old SD (Sprague-Dawley) rats followed by 8% O2 + 92% N2 for 2h. Totally 40 rats were randomly divided into 5 groups including sham-operated group, HIBD control group, HBO treated group, NGF treated group and NGF + HBO treated group. The learning and memory ability of these rats was evaluated by Morris water maze at 30 days after birth, and sensory motor function was assessed by experiments of foot error and limb placement at 42 days after birth. The escape latency of HBO treated group, NGF treated group and NGF + HBO treated group was shorter than that of HIBD control group (p<0.01) and longer than that of sham-operated group. The piercing indexes of 3 treated groups were higher than that of HIBD control group (p<0.01). Hyperbaric oxygen and nerve growth factor treatments may improve learning and memory ability and sensory motor function in neonatal rats after hypoxic ischemic brain damage.

Highlights

HIBD, common in clinical, is mainly caused by neonatal hypoxia and asphyxia in the perinatal period[1,2,3]
Some achievements have been made in neonatal asphyxia recovery, but no important improvement has been gained in the prognosis of HIBD5
HBO therapy helps to the protection and self-recovery of the affected brain[8] and protects brain from ischemia injury by inhibition of mitochondrial apoptosis[9]

Summary

Introduction

HIBD (hypoxic ischemic brain damage), common in clinical, is mainly caused by neonatal hypoxia and asphyxia in the perinatal period[1,2,3]. Neuron apoptosis, significant in the pathological process of HIBD, leads to the permanent neurological death or damage in newborns[3,5]. HBO therapy has a neuroprotective effect in hypoxic-ischemic rats and has no side effects in HIBD neonatal rats[10,11,12,13]. HBO therapy has neuroprotective effect by promoting brain cell proliferation[14] or by anti-apoptosis and antioxidative stress[15]. It has been suggested that NGF can take neuroprotective, anti-apoptotic and antioxidative effect, and NGF may protect neuron from injury through these effects[17]. Many researchers have been performed about the roles of HBO and NGF in several aspects, but the effects of HBO and NGF on long-term neural behavior of neonatal rats have not been clearly explained

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