Abstract
Glycolysis is a metabolic pathway vital to the production of energy and some organisms rely on it solely to meet their energy requirements. It is also a central pathway in the metabolism of carbohydrates and a source of therapeutic targets against diabetes and cancer. Caffeoylquinic acids (CQAs) have been extensively studied for their role in the treatment and prevention of diabetes (and cancer) but their mechanisms of action remain mostly unknown. As such, molecular docking was used to find possible targets of CQAs in the glycolysis pathway. The molecular docking assays showed that CQAs were docked preferably to the Rossman fold (nicotinamide adenine dinucleotide — NAD(H) binding site) of oxidoreductases, that use NAD(H) as a cofactor, than to any other site. In-vitro assays were then performed using two NAD(H) dependent oxidoreductases from glycolysis (alcohol dehydrogenase and L-lactate dehydrogenase) in order confirm if CQAs would compete with the cofactor to inhibit the reaction. The results from these assays indicate that CQAs can act as both inhibitors and activators of NAD(H) dependent oxidoreductases of the glycolysis pathway.
Highlights
Glycolysis was the first major metabolic pathway to be fully understood (Lenzen, 2014)
Considering the docking results and the limited availability of diCQAs two enzymes of this type that were potentially inhibited by different Caffeoylquinic acids (CQAs) were chosen for the in-vitro assays
Most of the results from the kinetic assays showed that the regression of the reactions where CQAs were present intercepted the uninhibited reaction well inside the first quadrant of the Lineweaver-Burk plots
Summary
Glycolysis was the first major metabolic pathway to be fully understood (Lenzen, 2014). This pathway has many steps that lead to the catabolism of glucose and other hexoses into pyruvate (Pelicano et al, 2006; Bar-Even et al, 2012). The pathway diverges depending on the availability of oxygen. NADH (nicotinamide adenine dinucleotide) is converted to NAD+ (Pelicano et al, 2006; Bolaños et al, 2010; Bar-Even et al, 2012).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
