Effects of hydrogen-rich water on aging periodontal tissues in rats.

Takaaki Tomofuji,Manabu Morita,Yasumasa Endo,Kenta Kasuyama,Daisuke Ekuni,Yuya Kawabata,Tetsuji Azuma,Toshiki Yoneda,Mayu Yamane

doi:10.1038/srep05534

Abstract

Oxidative damage is involved in age-related inflammatory reactions. The anti-oxidative effects of hydrogen-rich water suppress oxidative damage, which may aid in inhibiting age-related inflammatory reactions. We investigated the effects of drinking hydrogen-rich water on aging periodontal tissues in healthy rats. Four-month-old male Fischer 344 rats (n = 12) were divided into two groups: the experimental group (hydrogen-rich water treatment) and the control group (distilled water treatment). The rats consumed hydrogen-rich water or distilled water until 16 months of age. The experimental group exhibited lower periodontal oxidative damage at 16 months of age than the control group. Although protein expression of interleukin-1β did not differ, gene expression of Nod-like receptor protein 3 inflammasomes was activated in periodontal tissues from the experimental group as compared with the control group. Drinking hydrogen-rich water is proposed to have anti-aging effects on periodontal oxidative damage, but not on inflammatory reactions in healthy rats.

Highlights

IntroductionThe anti-oxidative effects of hydrogen-rich water suppress oxidative damage, which may aid in inhibiting age-related inflammatory reactions
During aging, it is reported that increased production of reactive oxygen species (ROS) induces oxidative mitochondrial DNA damage, which can stimulate the activation of the Nod-like receptor protein (NLRP) 3 inflammasome in tissues[4]
Because NLRP3 inflammasomes serve as a platform for caspase-1 activation and subsequent proteolytic maturation of the potent pro-inflammatory cytokine interleukin (IL)-1b4,5, these indicate that oxidative mitochondrial DNA (mtDNA) damage can stimulate age-related inflammatory reactions in tissues

Summary

Introduction

The anti-oxidative effects of hydrogen-rich water suppress oxidative damage, which may aid in inhibiting age-related inflammatory reactions. Drinking hydrogen-rich water is proposed to have anti-aging effects on periodontal oxidative damage, but not on inflammatory reactions in healthy rats. During aging, it is reported that increased production of ROS induces oxidative mitochondrial DNA (mtDNA) damage, which can stimulate the activation of the Nod-like receptor protein (NLRP) 3 inflammasome in tissues[4]. We found that drinking hydrogen-rich water suppressed ligature-induced periodontal inflammation in a rat model[12]. These studies mainly focused on the effects of hydrogen-rich water on human disease status and animal disease models. It remains unclear what effects drinking hydrogen-rich water have over the course of a lifetime

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