Abstract
Objective To evaluate the effect of hydrogen-rich saline on the expression of phosphor-p38 mitogen-activated protein kinase (p-p38MAPK) during cerebral ischemia-reperfusion (I/R) in rats. Methods Seventy-two adult male Sprague-Dawley rats, weighing 220-250 g, were randomly divided into 3 groups (n = 20 each) using a random number table: sham operation group (group S), I/R group and hydrogen-rich saline group (group I/RH). Cerebral ischemia was induced in chloral hydrate-anesthetized rats by 2 h middle cerebral artery occlusion in I/R and I/RH groups.The artery was only exposed but not occluded in group S. At 3 days before operation and immediately after onset of reperfusion, hydrogen-rich saline (0.6 mmol/L) 10 ml/kg was intraperitoneally injected in group I/RH, while the equal volume of normal saline was given in S and I/R groups.Neurological deficits were blindly assessed and scored at the end of 24 h reperfusion.The animals were then sacrificed, and brains were removed for microscopic examination and for determination of the cerebral infarct size (by TTC), brain water content, cell apoptosis (by TUNEL), and expression of p38MAPk and phosphor-p38MAPK (p-p38MAPK) (by immunohistochemistry and Western blot). Apoptosis index was calculated. Results Compared with group S, neurological deficit score, apoptosis index, brain water content and cerebral infarct size were significantly increased, and the expression of p38MAPK and p-p38MAPK was up-regulated in I/R and I/RH groups.Compared with group I/R, neurological deficit score, apoptosis index, brain water content and cerebral infarct size were significantly decreased, and the expression of p38MAPK and p-p38MAPK was down-regulated in group I/RH.The pathological changes of cerebral tissues were significantly attenuated in group I/RH as compared with group I/R. Conclusion Hydrogen-rich saline can reduce cell apoptosis through inhibiting p-p38MAPK expression, thus attenuating cerebral I/R injury in rats. Key words: Hydrogen; Reperfusion injury; Brain; Mitogen-activated protein kinases
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