Abstract
Abstract To explain what effect hydrochloric acid, contained in the sample to be analyzed, gives upon the paper chromatograms of amino acids, RF values of some amino acids dissolved in hydrochloric acid of various concentrations were observed with different kinds of solvent. In case of n-butanol, hydrochloric acid was found to increase the RF value of each amino acid with an increase in the concentration of hydrochloric acid, while in isovalerianic acid, on the contrary, to decrease that. On the one hand, only the amino acids of lower RF values were affected by hydrochloric acid in the case of phenol. In butanol-acetic acid, on the other hand, some effect was also found, but in collidine-lutidine it was negligible. In all cases, the relation between thess effects and the diffused areas of hydrochloric acid on the chromatograms was observed with great interest.
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