Effects of humic acids and herbicides, and their combinations on the growth of tomato seedlings in hydroponics

Abstract

AbstractThe morphology and length of roots and shoots of tomato (Lycopersicon esculentum Mill.) seedlings grown on a nutrient medium for fourteen days in a controlled environment chamber were apparently not affected, whereas the dry matter content of roots was significantly enhanced when 200 mg L−1 of humic acid (HA) isolated from either a non‐amended soil or a sewage‐sludge‐amended soil was present in the nutrient medium. In contrast, the HA‐like fraction isolated directly from the sewage sludge caused, under the same conditions, extensive alterations of tomato morphology and a significant reduction of the length and dry weight of both shoots and roots. The presence in the nutrient medium of the herbicides alachlor or imazethapyr at concentrations of 1 and 0.01 mg L−1, respectively, caused a marked decrease of tomato root and shoot length and dry weight. Differently, the herbicide rimsulfuron at a concentration of 0.01 mg L−1 produced a slight decrease in shoot and root length and a slight increase in their dry weight. A combination of 200 mg L−1 soil HA and each of the herbicides alachlor, rimsulfuron and imazethapyr at concentrations of 1, 0.01 and 0.01 mg L−1, respectively, in the nutrient medium attenuated the growth depression of tomato shoots and roots observed in the presence of the herbicide alone. However, the simultaneous presence of sewage sludge HA and any herbicide in the nutrient solution caused negative synergistic effects on tomato growth. The volume of nutrient solution and the amount of electrolytes taken up by tomato plants during the growth experiments correlated highly significantly with the total plant dry weight. Tomato seedlings induced a pH decrease in the nutrient medium in all treatments except in those where sludge‐HA was present, either alone or in combination with any herbicide.