Effects of human umbilical cord mesenchymal stem cells derived from exosomes on migration ability of endometrial glandular epithelial cells.

Abstract

The present study aimed to investigate the effects of human umbilical cord mesenchymal stem cells (Huc-MSCs)-derived exosomes on the migratory abilities of endometrial glandular epithelial cells, and to evaluate the underlying mechanism from the perspective of epithelial-mesenchymal transition (EMT). Huc-MSCs were prepared from human umbilical cord, and eutopic endometrial glandular epithelial cells were isolated from patients with endometriosis. The exosomes derived from Huc-MSCs (Huc-MSCs-exo) were prepared using an exosome extraction kit. The endometrial glandular epithelial cells were randomly divided into two groups: Huc-MSCs-exo and control. Cell migratory ability was assessed and western blotting was used to detect the expression levels of EMT. The results of the present study demonstrated that Huc-MSCs-exo treatment significantly enhanced the migration of endometrial glandular epithelial cells from patients with endometriosis (P<0.05). The present study also demonstrated that treatment with Huc-MSCs-exo inhibited the expression levels of E-cadherin and promoted the expression levels of Vimentin and N-cadherin at both the mRNA and protein level. The results of the current study indicate that Huc-MSCs-exo enhance the migratory ability of endometrial glandular epithelial cells via promotion of EMT.