Abstract
The present study aimed to investigate the effects of human umbilical cord mesenchymal stem cells (Huc-MSCs)-derived exosomes on the migratory abilities of endometrial glandular epithelial cells, and to evaluate the underlying mechanism from the perspective of epithelial-mesenchymal transition (EMT). Huc-MSCs were prepared from human umbilical cord, and eutopic endometrial glandular epithelial cells were isolated from patients with endometriosis. The exosomes derived from Huc-MSCs (Huc-MSCs-exo) were prepared using an exosome extraction kit. The endometrial glandular epithelial cells were randomly divided into two groups: Huc-MSCs-exo and control. Cell migratory ability was assessed and western blotting was used to detect the expression levels of EMT. The results of the present study demonstrated that Huc-MSCs-exo treatment significantly enhanced the migration of endometrial glandular epithelial cells from patients with endometriosis (P<0.05). The present study also demonstrated that treatment with Huc-MSCs-exo inhibited the expression levels of E-cadherin and promoted the expression levels of Vimentin and N-cadherin at both the mRNA and protein level. The results of the current study indicate that Huc-MSCs-exo enhance the migratory ability of endometrial glandular epithelial cells via promotion of EMT.
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