Effects of human placenta mesenchymal stem cell transplantation on superoxide dismutase 1 and uncoupling protein-2 in the treatment of premature ovarian failure in rats

Xiping Xu ,Xin Cheng ,Zhenhua Ma ,Qiaoni Hou ,Huiming Ma ,Yantao He ,Dongmei Chen ,Kaiyue Zhou ,Yanrong Wang ,Chaoqun Liu ,Xiang Li ,Xiuying Pei

doi:10.3760/cma.j.issn.2096-2916.2018.02.003

Abstract

Objective To investigate the effects of human placenta mesenchymal stem cell (hPMSC) transplantation on superoxide dismutase 1 (SOD1) and uncoupling protein-2 (UCP-2) in the treatment of premature ovarian failure in rats. Methods Sixty SD female rats with normal estrous cycle were randomly divided into four groups (n=15): blank control group, POF model group, treatment control group, and hPMSC transplantation group. It was determinated the ROS levels and 8-OHdG of serum by ELISA method. The ovarian tissue fibrosis was detected by VG staining method; The expression of SOD1 and UCP-2 protein was detected by Western blotting and immunohistochemical staining. Results Serum FSH level in the model group [(10.60±1.0) IU/L] was significantly higher than that in blank control group [(5.83±0.92) IU/L] (P<0.01), while E2 and AMH levels in the model group [(35.52±10.27) ng/L, (2 090.6±397.5) ng/L] were significantly lower than those in blank control group [(65.62±3.76) ng/L, (3 636.39±204.46) ng/L] (P<0.01). The count of the atretic follicle in hPMSC transplantation group (5.36±1.11) was lower than that in treatment control group (8.01±2.22) and POF model group (11.21±1.69) (P<0.05). Compared with the blank control group, the serum ROS was increased in the model group and the treatment control group (P<0.05); compared with the treatment control group, there was a significant change in ROS and 8-OHdG in the hPMSC transplantation group (P<0.05). VG staining showed that the ovarian tissue fibrosis in the model group was obvious, and after hPMSC transplantation there was a significant reduction compared with that in the treatment control group. The expression of SOD1 and UCP-2 protein has a statistical decrease in the hPMSC transplantation group compared with the treatment control group (P<0.05). Conclusion hPMSC transplantation may regulate the mitochondrial ROS synthesis and decrease oxidative stress by decreasing the expression of SOD1 and UCP-2 in rats. Key words: Human placenta mesenchymal stem cell (hPMSC); Premature ovarian failure (POF); Superoxide dismutase 1 (SOD1)/uncoupling protein 2 (UCP-2); Rats

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