Effects of human epidermalgrowth factor receptor-2/neu small interfering RNA-mediated gene silencing on cell proliferative activity in human meningiomas

Abstract

Objective To investigate the effects of human epidermal growth factor receptor-2 (Her-2)/neu gene on cell proliferative activity in human meningiomas. Methods The surgicall resected fresh samples from 5 cases of human meningiomas with Her-2 overexpression were selected,and cells were primarily cultured.The expression of Her-2 mRNA and protein was detected by reverse transcriptase -polymerase chain reaction(RT-PCR)and Western blotting respectively.Methyl thiazol tetrazolium (MTT)assay was employed to examined the cell viability.The cell cycle distribution was measured by flow cytometry. Results At 72nd h after transfection, as compared with the silencing group and the negative control group, the Her-2 m RNA and protein expression was reduced by 56. 3% and 52. 7% respectively(P< 0. 05). At 72nd h after transfection, the proliferation inhibition rate was 50. 08%.The proportion of cells in G0/G1 phase was increased by 18. 11%, and that in S phase decreased by 39. 34%(P< 0. 05). Conclusion Her-2 small interfering RNA(siRNA)-mediated gene may inhibit the proliferative ablity of human meningiomas. Key words: Meningioma; Human epidermalgrowth factor receptor-2; Small interfering RNA; Proliferation