Abstract
Colorectal cancer (CRC) is one of the most common gastrointestinal cancers in the world, and its incidence is increasing all over the world including China. In recent years, research data show that some miRNAs are differentially expressed in cancer tissues, and their expression is closely contributed with the prognosis of CRC. Microarray technology was used, and 179 miRNAs were screened out with significantly altered expression in CRC tissues compared with adjacent tissues. The expression of mir-145-5p in tumor tissues was 3.48 times lower than that in normal tissues. Using bioinformatics technology and network resource prediction, we found that mir-145-5p had a potential target gene relationship with msln gene. Then, qRT-PCR was used to validate the expression level of mir-145-5p and msln mRNA in CRC and paracancerous tissues. The results showed that msln mRNA was higher than in normal tissues, while mir-145-5p was lower, with statistically significant difference (P < 0.01, n = 3). Furthermore, the expression of msln protein in CRC and normal colorectal tissues was detected by protein mass spectrometry (MRM) (n = 3) and immunohistochemistry in a total case of 30 colorectal cancer tissues and normal tissues. Result showed that the positive expression of msln in CRC was higher than that in normal colorectal tissues, 1.38e-6 and 1.89e-6, respectively (P < 0.01, n = 3). Furthermore, in 48 h RTCA real-time monitoring experiment, mir-145-5p showed inhibitory effect on the proliferation of colo320 cells stimulated by msln. This study demonstrated that msln is a target gene of mir-145-5p in CRC. Besides, mir-145-5p negatively regulates the proliferation of CRC colo320 cells through downregulating msln gene expression in CRC colo320 cells.
Highlights
Colorectal cancer (CRC) is one of the most common gastrointestinal malignant tumors in the world, and incidence is increasing every year in many countries including China [1]
The data from the clustering analysis please refers to the file “Array QA/C lustering_analysis” and the cluster diagram “cluster_data.gif” which contains gene names. 179 differentially expressed genes were examined through cluster analysis of the quality of tumor tissue HJ (HJ1, HJ2, HJ3) and normal tissue HJC (HJC1, HJC2, HJC3) arrays in colorectal cancer (Figures 1(a) and 1(b))
Tumor cell metastasis is usually accompanied by regulation disorder of oncogene and tumor suppressor
Summary
Colorectal cancer (CRC) is one of the most common gastrointestinal malignant tumors in the world, and incidence is increasing every year in many countries including China [1]. The diagnosis and treatment level of colorectal cancer has been continuously improved, its five-year survival rate has not been significantly improved. It is a hot research topic to further clarify the exact molecular mechanism of colorectal cancer development [2, 3]. The exact mechanism for the development of colorectal cancer has not yet been fully elucidated. In addition to lymphatic tube, blood flow transfer and local invasion, it can be planted in the abdominal cavity or spread along sutures and incisions. It is more common in middle-aged men, from adenoma polyp cancer, the onset of younger age [5, 6]. The medical community hopes to find a new treatment method that can more effectively improve the effective cure rate of colorectal cancer [7]
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