Abstract
BackgroundAlthough discrepancy in the specific traits and ecological characteristics of Bemisia tabaci between species are partially attributed to the B. tabaci-associated bacteria, the factors that affect the diversity of B. tabaci-associated bacteria are not well-understood. We used the metagenomic approach to characterize the B. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria.Methodology and ResultsTo investigate the effects of the host plant and a virus, tomato yellow leaf curl virus (TYLCV), on the bacterial communities of B. tabaci sibling species B and Q, we analyzed the bacterial communities associated with whitefly B and Q collected from healthy cotton, healthy tomato, and TYLCV-infected tomato. The analysis used miseq-based sequencing of a variable region of the bacterial 16S rDNA gene. For the bacteria associated with B. tabaci, we found that the influence of the host plant species was greater than that of the whitefly cryptic species. With further analysis of host plants infected with the TYLCV, the virus had no significant effects on the B. tabaci-associated bacterial community.ConclusionsThe effects of different plant hosts and TYLCV-infection on the diversity of B. tabaci-associated bacterial communities were successfully analyzed in this study. To explain why B. tabaci sibling species with different host ranges differ in performance, the analysis of the bacterial community may be essential to the explanation.
Highlights
The sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a complex of species that contains at least 36 morphologically indistinguishable species [1,2,3,4] that cause considerable damage to a wide range of agricultural, fiber, vegetable, and ornamental crops through both direct feeding and vectoring of geminiviruses such as tomato yellow leaf curl virus (TYLCV) [2, 5]
To investigate the effects of the host plant and a virus, tomato yellow leaf curl virus (TYLCV), on the bacterial communities of B. tabaci sibling species B and Q, we analyzed the bacterial communities associated with whitefly B and Q collected from healthy cotton, healthy tomato, and TYLCV-infected tomato
For the bacteria associated with B. tabaci, we found that the influence of the host plant species was greater than that of the whitefly cryptic species
Summary
The sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a complex of species that contains at least 36 morphologically indistinguishable species [1,2,3,4] that cause considerable damage to a wide range of agricultural, fiber, vegetable, and ornamental crops through both direct feeding and vectoring of geminiviruses such as tomato yellow leaf curl virus (TYLCV) [2, 5]. The specific traits and ecological attributes of the species in the complex are related to the B. tabaci-associated bacteria, in part. The symbioses between B. tabaci and endosymbionts are well-documented [6,7,8], but the factors that affect the diversity of other bacteria associated with B. tabaci are not well-understood. The bacteria community associated with B. tabaci is a mix of mutualistic, pathogenic, and commensal bacteria. The primary symbionts compensate for the insufficient nutrients that B. tabaci obtains from a restricted diet of plant phloem [9]. Discrepancy in the specific traits and ecological characteristics of Bemisia tabaci between species are partially attributed to the B. tabaci-associated bacteria, the factors that affect the diversity of B. tabaci-associated bacteria are not well-understood. We used the metagenomic approach to characterize the B. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria
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