Abstract
MII mouse oocytes in 1 and 1.5 M ethylene glycol(EG)/phosphate buffered saline have been subjected to rapid freezing at 50 °C/min to −70 °C. When this rapid freezing is preceded by a variable hold time of 0–3 min after the initial extracellular ice formation (EIF), the duration of the hold time has a substantial effect on the temperature at which the oocytes subsequently undergo intracellular ice formation (IIF). For example, in 1 M EG, the IIF temperatures are −23.7 and −39.2 °C with 0 and 2 min hold times; in 1.5 M EG, the corresponding IIF temperatures are −29.1 and −40.8 °C.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
