Abstract
SUMMARY The effects of chemical fixatives such as the aldehydes, as well as ethanol, on the insulin content of the rat pancreas were studied. Although there was some loss of insulin after the use of all the fixatives investigated it was found that glutaraldehyde preserved the greatest amount of the hormone at both room and low temperature. Insulin was still preserved in the pancreas after glutaraldehyde fixation even after the complete preparation of the tissue for histology or electron microscopy. The insulin extracted from the pancreas after fixation in formaldehyde was still biologically active and it enhanced the formation of glycogen in the rat diaphragm.
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