Effects of Histamine on Survival and Immune Parameters of the Chinese Mitten Crab,Eriocheir sinensis

Abstract

ABSTRACT In the current study, we examined the effects of histamine on survival and immune parameters of the Chinese mitten crab, Eriocheir sinensis. The crabs infected with the common bacteria pathogen Aeromonas hydrophila were injected with 0 µg histamine/g/crab, 1 µg histamine/g/crab, 50 µg histamine/g/crab, or 100 µg histamine/g/crab, and their survival after 96 h was recorded. The results showed that histamine alone had no influence on crab survival but can decrease survival of the crabs challenged with A. hydrophila after 36 h. In addition, the survival of the crabs injected with the high level (100 µg/g) of histamine was significantly greater than that of the lower level (50 µg/g) after 60–96 h (P < 0.05), but not different from that of the control. Immune parameters of the hemolymph in crabs, such as the total hemocyte count (THC), phenoloxidase (PO), acid phosphatase (ACP), alkaline phosphatase (AKP), and superoxide dismutase activity (SOD), were measured at 6 h, 12 h, and 24 h after histamine in...