Effects of high intensity interval training (up & downward running) with BCAA/nano chitosan on Foxo3 and SMAD soleus muscles of aging rat

Abstract

AimsThe aim of this study was investigate the effects of 8 weeks of high intensity interval training (HIIT, up & downward running) with BCAA/nano chitosan on Foxo3 and SMAD soleus muscles of aging rats. Main methodsIn this experimental study thirty male rats were randomly divided into six groups of control, BCAA with Nano chitosan (Supplement, (Sup)), upslope running, downslope running, upslope running+Sup, and downslope running+Sup that each groups consist of 6 rats. The exercise training was performed HIIT 8 weeks 3 session per weeks with incrementally intensity 12 to 52 m/m in 7sets (Slop 0 to 15o) during 8 weeks. BCAA coated with chitosan nanoparticles (84 mg/kg) and gavage to supplementation groups, 3 days per weeks for eight weeks. The animals were feed with standard rat chow (Normal diet, 2.87 kcal/g, 15% of energy from fat). At the end of protocol the rat was sacrifice and soleus muscle was fix and frieze for IHC with H&E and gene expression analysis. Key findingsThe results of this study showed that Foxo3 gene expression in the Upslope running + Sup and Downslope running + Sup groups showed a significant decrease (p ≤ 0.05) compared to the control group. The mRNA of Smad also showed that only the Upslope running + Sup group had a significant decrease compared to the control group (p ≤ 0.05). SignificanceIt seems that, BCAA/nano chitosan supplementation along with exercise training in a variety of ways (Up & down slope running) can control the damage caused by Foxo3 and Smad transcription factors. That, control of these factors can minimize age-related atrophy.