Effects of high hydrostatic pressure on the pituitary–gonad axis in the European eel, Anguilla anguilla (L.)

Abstract

European silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the European continent to their spawning area in the Sargasso Sea. Tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us to hypothesise that high hydrostatic pressure (HP) influences the induction of eel reproduction. We subjected female and male silver eels to 101ATA for 3 and 7 weeks, respectively, in a hyperbaric chamber equipped with a freshwater recirculation system. In comparison with control eels kept at 1 ATA, HP effects were tested against the messenger RNA levels of pituitary gonadotropins (LHβ, FSHβ) using quantitative real-time RT-PCR. The effects of HP on gonadal activity were estimated by measuring gonadosomatic index, oocyte diameter and plasma levels of vitellogenin (Vtg) and sex steroids (E 2, 11-KT). At the pituitary level, LHβ expression tended to increase while FSHβ expression decreased in both sex, leading to an increase in the LHβ/FSHβ ratio. This suggests a differential effect of HP on the expression of the two gonadotropins. In females submitted to HP, we observed a significant increase in oocyte diameter and plasma levels of 11-KT and E 2. A similar trend was observed for 11-KT plasma levels in males. In females, Vtg plasma levels also significantly increased, reflecting the stimulatory effect of sex steroids on hepatic vitellogenesis. Our results suggest that HP plays a specific and positive role in eel reproduction but additional environmental and internal factors are necessary to ensure complete sexual maturation.