Effects of high hydrostatic pressure on enzymes, phenolic compounds, anthocyanins, polymeric color and color of strawberry pulps

Abstract

Changes in activity of polyphenol oxidase (PPO), peroxidase (POD) and β-glucosidase, individual phenolic compounds other than anthocyanins, total phenols, monomeric anthocyanins, polymeric color and instrumental color of strawberry pulps were assessed after high hydrostatic pressure (HHP) (400-600 MPa 5-25 min(-1)) at room temperature. β-Glucosidase was activated by 4.7-16.6% at 400 MPa 5-25 min(-1) and inactivated by 8.0-41.4% at 500 or 600 MPa. PPO and POD were inactivated at all pressures, the largest reduction in activity being 41.4%, 51.5% and 74.6%, respectively. The individual phenolic compounds and total phenols decreased at 400 MPa, but total phenols increased at 500 or 600 MPa. However, the monomeric anthocyanins, polymeric color and redness (a*) exhibited no change. HHP induced a decrease in lightness (L*) and an increase in yellowness (b*) at 400 MPa, but no significant alteration in L* value and b* value at 500 or 600 MPa was observed; this was attributed to higher residual activity of PPO, POD and β-glucosidase at 400 MPa. Total color difference (ΔE) was ≥5 at 400 MPa and ≤3 at 500 or 600 MPa. HHP effectively retained anthocyanins, phenolic compounds and color of strawberry pulps, and partly inactivated enzymes.