Abstract
Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. Sub-microsecond regime of electric pulses displays unique electrophysical features operative in cells and membranes. Recently, MHz compression of nanosecond pulses electric fields (nsPEFs) bursts proved to enhance the effectiveness of the therapy. High morbidity of prostate cancer (PCa) and risk of overtreatment associated with this malignancy call for new minimal-invasive treatment alternative. Herein we present the in vitro study for developing applications based on this new technology. In this study, we used flow cytometric analysis, cell viability assay, caspase activity analysis, wound healing assay, confocal microscopy study, and immunofluorescence to investigate the biological effect of high-frequency nsPEFs on PCa cells. Our results show that high-frequency nsPEFs induces the permeabilization and cell death of PCa cells. The cytotoxicity is significantly enhanced in MHz compression of pulses and with the presence of extracellular Ca2+. High-frequency nsPEFs trigger changes in PCa cells’ cytoskeleton and their mobility. The presented data show a therapeutic potential of high-frequency nsPEFs in a PCa setting. The sub-microsecond regime of pulses can potentially be applied in nanosecond electroporation protocols for PCa treatment.
Highlights
Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors
It was observed that nanosecond pulsed electric fields preferably affect cells interior acting on intracellular b iomembranes[3]
The permeabilization rate of prostate cancer (PCa) cells depends on the electric field intensity and frequency of pulsed electric fields (PEFs)
Summary
Electroporation with pulsed electric fields show a potential to be applied as an experimental focal therapy of tumors. High morbidity of prostate cancer (PCa) and risk of overtreatment associated with this malignancy call for new minimal-invasive treatment alternative. Our results show that high-frequency nsPEFs induces the permeabilization and cell death of PCa cells. The sub-microsecond regime of pulses can potentially be applied in nanosecond electroporation protocols for PCa treatment. Intense pulsed electric fields (PEFs) can be applied to permeabilize b iomembranes[1]. This phenomenon is known as electroporation and found its use among others in the minimal-invasive treatment of different types of cancer. Electroporation constitutes a new Scientific Reports | (2021) 11:15835
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