Effects of Hibiscus Sabdariffa Calyces Aqueous Extract on the Antihypertensive Potency of Captopril in the Two-Kidney-One-Clip Rat Hypertension Model.

Shinta Ayu Nurfaradilla,Yahdiana Harahap,Fadlina Chany Saputri

doi:10.1155/2019/9694212

Abstract

Hibiscus sabdariffa aqueous extract (HS) is often used as complementary therapy for hypertension. However, some studies have shown that coadministration with a conventional antihypertensive drug can affect drug potency. We compared the effects of HS plus captopril (CAP) coadministration to HS and CAP administration alone on blood pressure and renin-angiotensin-aldosterone system (RAAS) biomarkers in the rat two-kidney-one-clip (2K1C) model of hypertension. Male Sprague Dawley rats were randomly divided into seven groups (n=6/group), a normal control (SHAM) group, and six 2K1C groups. In 2K1C animals, hypertension was induced using a stainless microclip (inner diameter of 0.20 mm). Four weeks after 2K1C surgery, blood pressure was significantly higher than in the SHAM group. Then, model rats were randomly divided into negative control (2K1C, no treatment), positive control (4.5 mg captopril/200 g body weight [BW] orally [p.o.]), HS alone (30 mg/200 g BW; p.o.), and 3 co-treatment groups receiving HS (15, 30, or 60 mg/200 g BW; p.o.) plus 4.5 mg/200 g BW captopril. The treatments were performed for two weeks. Blood pressure was significantly reduced by all the drug treatments to near the level of SHAM controls. Plasma renin level, serum angiotensin converting enzyme (ACE) activity, and plasma angiotensin II level were also significantly elevated in the 2K1C group compared to the SHAM group. Both serum ACE activity and plasma angiotensin II level were significantly reduced to near SHAM group levels by all the drug treatments. Hibiscus sabdariffa aqueous extract alone can reduce blood pressure. This extract appears could be used as a supplement with captopril but may not provide any additional benefit.

Highlights

Hypertension increases the risks of mortality from cardiovascular, renal, and neurological diseases and, has become a major global health concern [1]
Effects of Hibiscus Sabdariffa Aqueous Extract, Captopril, and Cotreatment on Blood Pressure in K C Model Rats. Both systolic and diastolic blood pressure were rose progressively over 4 weeks after surgery in 2K1C model rats compared to the SHAM group (p < 0.05, Figure 1)
Both systolic and diastolic blood pressure were significantly reduced in all the drug treatment groups (CAP, Hibiscus sabdariffa aqueous extract (HS), CD 1, CD 2, and CD 3) compared to the untreated 2K1C group (p < 0.05, Figure 2)

Summary

Introduction

Hypertension increases the risks of mortality from cardiovascular, renal, and neurological diseases and, has become a major global health concern [1]. Effective management of hypertension often requires combination therapy [2, 3]. Less than 40% of patients who combine herbal preparations with conventional antihypertensive drug therapy do not inform their doctor and so may be unaware of certain deleterious side effects or herbs-drug interactions. Hibiscus sabdariffa is widely used as a herbal antihypertensive [5, 6] and frequently as complementary therapy [7, 8]. The antihypertensive efficacy of Hibiscus sabdariffa extract has been documented in both preclinical studies and randomized control trials [2, 5, 8, 9]. Anthocyanin and quercetin were the components in Hibiscus sabdariffa extract responsible for the antihypertensive effect [2, 5, 9, 10]. Several studies have found that coadministration of Evidence-Based Complementary and Alternative Medicine

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