Abstract
Purpose: Sporadic Alzheimer’s disease (AD) accounts for over 95% of cases. Possible mechanisms of AD such as inflammation and oxidative stresses in the brain motivate researchers to follow many therapies which would be effective, especially in the early stages of the disease. IMOD, the herbal extract of R. Canina, T. Vulgare and U. Dioica plant species enriched with selenium, has anti-inflammatory, immunoregulatory and protective effects against oxidative stress.Methods: In this study three AD-related genes, DAXX, NFκβ and VEGF, were chosen as candidate to investigate the neuroprotective effect of the extract by comparing their expression levels in the hippocampus of rat model of sporadic AD, using qPCR in the herbal-treated and control groups. The therapeutic effects on learning and memory levels were evaluated by Morris Water Maze (MWM) test.Results: Gene expression results were indicative of significant up-regulation of Vegf in rat’s hippocampus after treatment with the herbal extract comparing to model group (P-value= 0.001). The MWM results showed significant changes in path length and time for finding the hidden platform in all groups during test and the same change in the treated comparing to the control group in memory level.Conclusion: It could be concluded that the herbal extract may have significant effect on gene expression but not on behavioral level.
Highlights
Sporadic Alzheimer's disease (AD) is a complex disorder which both genetic and environmental risk factors are involved.[1]
In this study according to the importance of molecular mechanisms of Alzheimer’s disease (AD) such as inflammation and oxidative stresses in the brain, the neuroprotective effect of this herbal extract was investigated by evaluating the expression levels of the three AD-related genes, Daxx, Nfκβ and Vegf, in the hippocampus of rat model of Sporadic Alzheimer's disease (SAD) using qPCR in treated and untreated groups
Behavioral Results After assessing the learning and memory level changes by Morris Water Maze test, as it is obvious in Figure[1], the results showed a significant reduction in swimming distance and time for finding the hidden platform during five days in all groups except alcohol group; no significant change was observed in the herbal-treated
Summary
Sporadic Alzheimer's disease (AD) is a complex disorder which both genetic and environmental risk factors are involved.[1]. Methods: In this study three AD-related genes, DAXX, NFκβ and VEGF, were chosen as candidate to investigate the neuroprotective effect of the extract by comparing their expression levels in the hippocampus of rat model of sporadic AD, using qPCR in the herbal-treated and control groups.
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