Effects of heat stress on photosynthetic characteristics and chloroplast ultrastructure of a heat-sensitive and heat-tolerant cultivar of wucai (Brassica campestris L.)

Abstract

The hot climate of southern China from late summer to early fall is one of the major factors limiting the yield and quality of wucai (Brassica campestris L.). To study the effects of high temperature on the growth and development of wucai, two cultivars (heat-sensitive ‘WS-6’ and heat-tolerant ‘WS-1’) were subjected to three heat stress treatments for 5 days. The two cultivars were evaluated for plant growth, photosynthetic characteristics, oxidative damage, and activities of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Heat stress treatments higher than 30 °C resulted in significant changes for both cultivars in plant growth (plant height, stem diameter, and single plant weight) and photosynthetic performance [net photosynthetic rate (PN), stomatal conductance (gs), intercellular CO2 concentration (Ci), and leaf transpiration rate (E)]. The chlorophyll (Chl) content was markedly reduced by heat stress, inhibiting photochemical activity. However, larger decreases in growth and photosynthetic parameters occurred under heat stress in WS-6, compared with WS-1. Furthermore, microscopy also revealed greater severity of damage to the photosynthetic apparatus and membrane system of WS-6 under heat stress, compared with WS-1. These effects were probably due to a greater accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) in WS-6, relative to WS-1. Moreover, higher photochemical efficiency of photosystem II (PSII) and greater activities of antioxidant enzymes were present in WS-1, compared with WS-6. Our results indicated that the extent of heat stress damage in wucai was cultivar dependent. The heat-tolerant cultivar WS-1 had a greater capacity for self-protection and self-regulation under heat stress.