Abstract

Using multi-trait animal model BLUP, selection was conducted over seven generations for growth rate (DG), real-time ultrasound loin-eye muscle area (LEA), backfat thickness (BF), and intramuscular fat content (IMF) to develop a new line of purebred Duroc pigs with enhanced meat production and meat quality. This study was intended to investigate the relationship between restriction fragment length polymorphism (RFLP) of a heart fatty acid-binding protein (H-FABP) gene and intramuscular fat content (IMF) of this Duroc purebred population. The present experiment examined the RFLP of 499 slaughtered pigs. The DNA was separated from the blood or ear tissue of the pigs, which were slaughtered at 105 kg of body weight. Intramuscular fat content of the longissimus muscle was measured using chemical analysis. A significant difference was detected in the breeding value of IMF among the H-FABP PCR RFLP genotypes. The AA genotype has a significantly larger positive effect on the IMF breeding value than do the Aa and aa genotypes for the MspI RFLP. In addition, the DD genotype has a significantly greater positive effect on IMF breeding value than the Dd and dd genotypes for the HaeIII RFLP. For the HinfI RFLP, the hh genotype has a significantly larger positive effect on IMF breeding value than the HH genotype. Multiple regression analysis was performed using the IMF breeding values as the dependent variable and the three H-FABP genotypes as independent variables. Results revealed that the contribution of the genotypes to variation in IMF breeding values was approximately 40%. These results demonstrated that H-FABP RFLPs affect IMF in this Duroc population.

Highlights

  • Meat quality traits, in addition to meat production traits, have become important traits for selection in pig breeding

  • The desired gains for DG, loin-eye muscle area (LEA), and backfat thickness (BF) were not achieved, but the average breeding value for intramuscular fat content (IMF) at the seventh generation (1.20%) became greater than the initially desired gain (0.7%)

  • Present results indicate that AA, DD, and hh genotypes have the largest effects on IMF; but Gerbens and coworkers concluded that the aa, dd, and HH genotypes showed the greatest effects

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Summary

Introduction

In addition to meat production traits, have become important traits for selection in pig breeding. Selection for meat production and meat quality (intramuscular fat content: IMF) traits was conducted using multi-trait animal model BLUP in Duroc pigs over seven generations (Suzuki et al, 2005). The average breeding value of IMF at the seventh generation (1.20%) was greater than the initially desired gain (0.7%). To improve meat quality traits such as IMF, via traditional methods of selection, it would be necessary to slaughter numerous pigs.

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