Effects of Haima Duobian Pill in a Rat Model of Kidney Yang Deficiency Syndrome.

Yijia Zeng,Xiaorui Zhang,Li Yuan,Jin Wang,Yuanyuan Ren,Funeng Geng,Qinwan Huang,Tingna Li,Luiz Felipe Domingues Passero

doi:10.1155/2021/6696234

Abstract

Objective Modern research shows that Haima Duobian pill (HDP) can relieve the kidney yang deficiency syndrome (KYDS), but the mechanism is still unclear. The aim of this work was to study the effects of HDP in a rat model of KYDS. Materials and Methods The network pharmacology methods were used to predict the therapeutic effects of Haima Duobian pill. Adenine was used to establish the rat model of kidney yang deficiency syndrome. The general physical signs of rats were observed after different doses of Haima Duobian pill (HDP) were given. Serum cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone (T), estradiol (E2), and gonadotropin-releasing hormone (GnRH) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits. Then, the histopathologic changes and sperm activity were detected. Results HDP could improve the general signs of kidney yang deficiency syndrome rats. After the rats were treated with HDP, the expression of cGMP and E2 was significantly inhibited and the expression of cAMP and T was significantly increased. The pathological damage of testis, epididymis, and seminal vesicle was alleviated, and the sperm activity was improved. Conclusion For adenine-induced kidney yang deficiency syndrome in rats, HDP had a significant therapeutic effect.

Highlights

Kidney yang deficiency syndrome (KYDS), a diagnostic pattern in Chinese medicine, has clinical features similar to those of the glucocorticoid withdrawal syndrome [1]
Kidney yang deficiency syndrome (KYDS), a metabolic disease caused by a neuroendocrine disorder, was recorded first in an early systematic and theoretical monograph existing in China, “Neijing” [7]. e theory of traditional Chinese medicine (TCM) proposes that the physiological function of the body would be at a low level in KYD syndrome
We observed that KYDS rat model showed lower body weight growth rate and body temperature than normal rats, and the model group rats generally had the symptoms of emaciation, curling and arching back, chilly limbs, yellow and loose body hair, mental weakness, slow response, increased drinking water, reduced diet, and polyuria. ese results are similar to those reported in the literature

Summary

Introduction

Kidney yang deficiency syndrome (KYDS), a diagnostic pattern in Chinese medicine, has clinical features similar to those of the glucocorticoid withdrawal syndrome [1]. It is a metabolic disease caused by a neuroendocrine disorder [2]. Modern research has found that the dysfunction of hypothalamicpituitary-gonadal axis (HPG axis) is the basis of KYDS, and the HPG axis consists of three levels: hypothalamus, pituitary, and gonads (testes in the male and ovaries in the female) [4]. Based on the HPG axis, this study will explore the effect of HDP on spermatogenesis in rats with KYDS

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Discussion

Conclusion