Effects of H-2 complex and non-H-2 background on urethane-induced chromosomal aberrations in mice

Abstract

The incidence of in vivo urethane-induced chromosomal aberrations was examined in H-2 congenic strains of mice with B10 and A backgrounds. Chromosome analysis of bone-marrow cells could divide 7 lines of A.H-2 congenic strains into 2 groups: one with a higher frequency of chromosomal aberrations such as in A/Wy (haplotype H-2 a), A/J (H-2 a), A.AL (H-2 a1) and A.TL (H-2 t1), and the other consisting of A.TH (H-2 t2), A.CA (H-2 f), A.BY (H-2 b) and A.SW (H-2 s). The same tendency was also observed in the spleen cells. Among B10.H-2 congenic mice, B10.A (H-2 a), B10.BR (H-2 k), B10.A(3R) (H-2 i3), B10.A(5R) (H-2 i5) and B10.S(9R) (H-2 t4) exhibited significantly higher rates of induced chromosomal aberrations than those in B10 (H-2 b), B10.S (H-2 s), B10.A(2R), (H-2 h2), B10.A(4R) (H-2 h4) and B10.S(7R) (H-2 t2). To determine the effect on non-H-2 genetic backgrounds on urethane-induced chromosomal aberrations, 4 pairs of strains which have the same H-2 haplotypes, such as in B10 vs. A.BY (H-2 b), B10.A vs. A/Wy (H-2 a), B10.S vs. A.SW (H-2 s), and B10.S(7R) vs. A.TH (H-2 t2), were compared. The strains with a B10 background exhibited significantly higher frequencies of deletions and lower frequencies of exchanges than the strains with an A background. These data suggested that at least two genes are involved in the regulation of urethane-induced chromosomal aberrations in mice, one of which is mapped between the S and D regions in the H-2 complex, and another not belonging to H-2.