Effects of growth hormone on hormones and morphology of ovary and endometrium in polycystic ovary syndrome rats

Abstract

Objective To explore the effect of recombinant human growth hormone(rhGH)on polycystic ovary syndrome(PCOS)and its mechanism through observing the sex hormone, the influence of ovarian and endometrial tissue morphology of PCOS rat models. Methods All 60 6 weeks of clean female SD rats were chosen.20 of them were divided into blank control group, the rest 40 rats received intragastric administration of letrozole solution to establish PCOS rat model, the success of the model group rats were randomly divided into three groups: rhGH group, taie - 35(ethinylestradiol cyproterone)group and PCOS control group, then subcutaneous injection of rhGH for rhGH group 1 U/(kg·d), Diane-35 was given by lavage for taise-35 group[240 mg/(kg·d)]. PCOS control group and blank control group subcutaneous were injected with the same amount of saline, All rats were executed after treating 21 days.Application of enzyme-linked immune sobent assay(ELISA) method to detect the rat serum for follicle-stimulating hormone(FSH), luteinizing hormone(LH), progesterone(P), estradiol(E2), the expression level of testosterone(T), then on the ovarian and endometrial morphology analysis. Results Compared with PCOS group, rhGH rats serum hormone levels drop T, E2, P levels, differences were statistically significant(P 0.05), ovarian cystic expansion follicles decreased significantly, at all levels of follicle and corpus luteum number increased significantly, granular cell layer thickening, increased number of endometrial glands, clusters of arrangement, lumens partly visible to some creases and branch. Conclusion RhGH can improve the model of PCOS rats serum sex hormone levels, endometrial morphology, and ovarian oviposit function, so as to achieve the effect of treatment of PCOS. Key words: Polycystic ovary syndrome; Growth hormone; Ovary morpholog; Endometrial morphology