Effects of Grinding Methods on Physicochemical Properties of Crude Polysaccharides from Phellinus baumii

Tingting Li,Wanling Yang,Linjun Chen,Yan Yang

doi:10.1051/e3sconf/20197802004

Tingting Li, Wanling Yang + Show 2 more

Open Access

https://doi.org/10.1051/e3sconf/20197802004

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Abstract

The physicochemical properties of Phellinus baumii polysaccharides extracted from the fruiting bodies by different grinding methods were studied in this paper. Compared with ordinary grinding methods, superfine grinding technology exerts significantly higher yields of crude polysaccharides, polysaccharide contents and β-Glucan contents of six samples. HPSEC-MALLS- RI analysis shows the molecular weight distribution of P.baummii crude polysaccharides, which indicates superfine grinding technique generates higher molecular weight fractions and larger polydispersities. The 6 kinds of crude polysaccharides have obvious effects on NO generation of RAW264.7 cells, which indicated that crude polysaccharides from P.baumii may have some immune-enhancing effects. Meanwhile, CW30 and FP30 show the best immune enhancing effects. However, CW50 and CW70 exert weaker immune-enhancing effects compared with FP50 and FP70.

Highlights

Phellinus baumii Pilát belonging to Basidiomycota, Hymenomycetes, Aphyllophorales, Hymenochaetaceae and Phellinus, is a perennial, yellowish brown rare medicinal fungus[1]
The research on superfine grinding technology is increasing with the development of new medicines, health product development, bioengineering, food processing, etc., which has been reported the superfine grinding is applied in the extraction of flavone in ginkgo leaves, Poria cocos polysaccharides, and functional properties of mushrooms, [10,11,12], but the effect of superfine grinding technology on the extraction of crude polysaccharides from Phellinus fruiting body and the physicochemical properties of polysaccharides has not been reported
This paper takes Phellinus super fine powder as the research object, compares the differences of physicochemical property and bioactivity of Phellinus fruiting body extracted from different grinding ways, it was found that the crude polysaccharide of Phellinus obtained after superfine grinding treatment, there is a significant increase in polysaccharide yield, polysaccharide content and β-glucan content

Summary

Introduction

Phellinus baumii Pilát belonging to Basidiomycota, Hymenomycetes, Aphyllophorales, Hymenochaetaceae and Phellinus, is a perennial, yellowish brown rare medicinal fungus[1]. Numerous studies have shown that superfine grinding technology can improve the dissolution rate of effective components in medicines, increase the bioavailability of the medicine and save the medicine. The research on superfine grinding technology is increasing with the development of new medicines, health product development, bioengineering, food processing, etc., which has been reported the superfine grinding is applied in the extraction of flavone in ginkgo leaves, Poria cocos polysaccharides, and functional properties of mushrooms, [10,11,12], but the effect of superfine grinding technology on the extraction of crude polysaccharides from Phellinus fruiting body and the physicochemical properties of polysaccharides has not been reported. This research selects the fruiting body of P.baumii to compare the effects of superfine grinding and ordinary grinding ways on the physicochemical properties of polysaccharide, and the bioactivites of polysaccharides extracted by grinded in two ways was compared, which provide basis for further research and utilization of Phellinus polysaccharide

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