Effects of Green, Oolong and Black Teas and Related Components on Proliferation and Invasion of Hepatoma Cells

Abstract

Employing both in vitro and ex vivo assay systems, we studied the effects of extracts from powdered green, oolong and black teas and related tea components on the proliferation and invasion of an ascites hepatoma cell line of AH109A. The AH109A cells were found to penetrate underneath the monolayer of primary cultured mesothelial cells isolated from Donryu rat mesentery in the presence of 10% calf serum. Each tea extractdose-dependently restrained this invasion and the AH109A proliferation. About 0.04% of each extract had strong inhibitory effects on the invasion and proliferation. When rat serum obtained after oral intubation of each tea extract was added to the culture media instead of calf serum at a concentration of 10%, the invasion of AH109 A was significantly inhibited as compared with the control rat serum. The proliferation of AH109A was also inhibited by the tea extract-loaded rat serum obtained after oral intubation of each extract. Furthermore, (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (EGG) and (-)-epigallocatechin (EGC) from green tea as well as the mixture of theaflavin and theaflavin gallates (TFs) from black tea were shown to be the most effective components against the invasion and proliferation of AH109A among all components screened.These results confirm that not only green tea but black tea, oolong tea and their related components also have an inhibitory effect on the invasion and proliferation of AH109A.KeywordsEpigallocatechin GallateEpicatechin GallateOral IntubationAH109A CellAscites Hepatoma Cell LineThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.