Abstract
This work evaluated the effect of grape juice, red wine and resveratrol in liver parameters of rats submitted to high-fat diet. Experimental model was conducted with groups of adult females Rattus norvegicus: control (CG); high-fat (HG); grape juice (JG); red wine (RW) and resveratrol solution (RG). The high-fat diet significantly altered hepatocytes and Kupffer cells in all treated groups. HG group presented severe steatosis followed hepatocyte ballooning and tissue damages. JG group minimized hepatic histological lesion caused by high-fat diet and WG group also induced steatosis and inflammation in hepatocytes, similar to HG. Still, resveratrol protected the tissue against fatty liver disease by reducing fat infiltration and inflammation, indicating possible therapeutic effects on the liver. Cell cycle analysis showed that HG promoted damage to the tissue, reducing the viable cell content and increasing apoptosis, even when associated with wine consumption or isolated resveratrol. However, JG protected the liver against cell damage generated by the diet. Consumption of grape juice, even associated with a high-fat diet, represents a promising protection of the liver against cellular damage, but red wine further affects the tissue, and resveratrol alone was able to reduce damage but did not minimize cellular damage to the liver.
Highlights
Diets play major role in both health care and disease progression (Pereira et al 2014, Milić et al 2014)
The consumption of resveratrol solution (RS) and grape juice (GJ) was higher than the red wine (RW) group, but despite the difference found in the consumption of beverages, all groups had similar energy consumption when we associated the feed consumption (Table II)
Considering that the high-fat diet is a common choice of part of the population and that its deleterious effects have been observed (Fontelles et al 2018, De Oliveira et al 2016), this study aimed at assessing the effects of grape juice, red wine and isolated resveratrol on rat liver fed a high-fat diet, with regard to the cell cycle, apoptosis and hepatocyte histology
Summary
Diets play major role in both health care and disease progression (Pereira et al 2014, Milić et al 2014). The Consumption of a high-fat diet is associated with increased body weight, metabolic changes, such as inflammation and oxidative stress, cell damage as well as chronic non-transmissible diseases involving various tissues such as the liver. Excessive intake of lipids induces increased flow and oxidation of fatty acids in the liver with increased fat deposition in hepatocytes and promoted inflammation and lipotoxicity contributing to cell malfunction, cell death and cell lesions (Cohen et al 2011). Associated with a high-fat diet, alcohol consumption is reported as an etiological factor for hepatic steatosis, as well as other drugs or toxins (Noureddin & Rinella 2015). Excessive fat deposition in the liver causes mitochondrial dysfunction and fatty acids oxidation produces high production of reactive oxygen species (ROS) as side product of that causes damage to cellular membranes, proteins, lipids and deoxyribonucleic acid (DNA) (Noureddin & Rinella 2015, Reynés 2015).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
