Abstract
Objective To investigate the effects of graft size on the survival of the graft.Methods F1 mice were bred by crossing male C57BL/6 and female BALB/c mice.Newborn C57BL/6 mice were infused with 1.5 × 107 F1 spleen cells(newborn-induced transplant tolerance,NTT).Clonal deletion of alloreactive T cells was measured by mixed lymphocyte reaction (MLR).Na(i)ve and NTT mice were transplanted with 0.5 cm2 and 1 cm2 skin grafts from F1 mice to evaluate their tolerance.Results Infusion of spleen cells from F1 mice significantly depleted alloreactive T cells from C57BL/6 mice,and reduced anti-graft reactivity.Naive mice rapidly rejected both 0.5 cm2 and 1 cm2 skin grafts with same process [(8.9 ± 1.0) days and (9.3 ± 1.0) days,P ＞ 0.05].The survival time of 1 cm2 skin grafts in NTT mice is higher than that of 0.5 cm2 skin grafts with (63 ± 28) vs (28 ± 16) days respectively (P ＜ 0.05).Long-term tolerance was observed in 1 cm2 but not in 0.5 cm2 skin grafts.Conclusion Enlarging the volume of the graft in conditions with reduced frequency of alloreactive T cells can prolong graft survival time. Key words: Graft size; Transplantation tolerance; Alloreactive T cells; Mice
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