Effects of Gonadectomy on Plasma Gonadotropins I and II in Coho Salmon,Oncorhynchus kisutch

Abstract

Studies of both salmon and trout have indicated that the levels of gonadotropins, GTH I and GTH II, in the pituitary and peripheral circulation vary during the reproductive cycle. To evaluate the possible feedback relationship between the gonads and pituitary GTH secretion, we studied the effects of bilateral gonadectomy on plasma levels of GTH I and GTH II in coho salmon,Oncorhynchus kisutch.During late spermatogenesis in males and late vitellogenesis in females, plasma GTH I levels increased significantly after gonadectomy, approximately 6- and 5-fold over presurgery levels at 3 and 14 days after surgery, respectively, and then declined to near presurgery levels by Day 17. No change in GTH I levels occurred in sham-operated fish. In all groups, GTH II levels were nondetectable and did not change significantly up to 17 days postsurgery. In males gonadectomized during spermiation, plasma GTH I levels increased significantly, approximately 10-fold over presurgery levels by 7 days postsurgery, and remained elevated thereafter. In contrast to the males in late spermatogenesis, the spermiating fish had detectable levels of GTH II (2–3 ng/ml), and significant elevations in plasma GTH II levels (approximately 60-fold) were observed 7 days after gonadectomy. These experiments demonstrate that the gonads exert negative feedback effects on secretion of both GTH I and GTH II, but the effect varies seasonally and the nature of the specific factor(s) from the gonads that inhibits and/or stimulates GTH production and secretion remains to be clarified.