Effects of gonadal steroids on pineal morphogenesis and cell differentiation of the embryonic quail studied under cell culture conditions

Abstract

Receptors for gonadal steroid hormones have been localized in the pineal glands of several vertebrate species. No studies, however, have reported on pineal morphogenesis and cell differentiation following hormonal application in vitro during avian embryonic development. Hormonal regulation of embryonic development is crucial in all vertebrate classes. Although gonadal hormones are known to affect organogenesis in avian embryos and chicks, we wanted to investigate whether gonadal steroids (testosterone and estradiol) have any effect on the morphogenesis and cell differentiation of the avian pineal gland. The steroid hormones had a stimulatory influence on pineal morphogenesis in vitro as evidenced from the radial arrangement of colony-forming cells and the subsequent formation of a follicular-like structure under dispersed-cell culture condition. Administration of testosterone in culture medium significantly promoted the numbers of cells that were positively stained for arginine vasopressin and tyrosine hydroxylase, while estradiol showed only a slight effect. Both of the two steroid hormones significantly decreased the numbers of cells positively stained for serotonin and melatonin. Melatonin released in the culture medium decreased in content within the 24 h following steroid treatment (supported by low immunoreactivity in cultured cells and low level released to the medium). These results clearly suggest active roles of gonadal steroid hormones on embryonic pineal morphogenesis and cell differentiation and its physiological activity as they do in adult animals.