Effects of glyphosate on germination, photosynthesis and chloroplast morphology in tomato

Abstract

The adverse effects of glyphosate herbicide on plants are well recognised, however, potential hormetic effects have not been well studied. This study aimed to use tomato as a model organism to explore the potential hormetic effects of glyphosate in water (0–30 mg L−1) and in compost soil (0–30 mg kg−1). The growth-promoting effects of glyphosate at concentrations of 0.03–1 mg L−1 in water or 0.03–1 mg kg−1 in compost were demonstrated in tomato for the first time. These hormetic effects were manifest as increased hypocotyl and radicle growth of seedlings germinated on paper towel soaked in glyphosate solution and also in crops which had been sprayed with glyphosate. Increased rates of photosynthesis (up to 2-fold) were observed in 4-week old crops when seeds were sown in compost amended with glyphosate and also when leaves were sprayed with glyphosate. The examination of chloroplast morphology using transmission electron microscopy revealed that the hormetic effects were associated with elongation of chloroplasts, possibly due to lateral expansion of thylakoid grana.