Effects of glycerin fructose combined with dexamethasone on the expressions of aquaporins-4 and matrix metalloproteinases-9 and neurological function in mice after cerebral hemorrhage

Abstract

Objective To assess the effects of glycerin fructose combined with dexamethasone on neurological function and the expressions of aquaporins (AQP)-4 and matrix metalloproteinases (MMP)-9 in mice after cerebral hemorrhage. Methods Totally 96 male mice(kunming) were randomly divided into the control group (n=24), the model group (n=24), dexamethasone treatment group (n=24), glycerin fructose combined with dexamethasone treatment group (combination group, n=24). The cerebral hemorrhage model was set up in the last three groups, which were given corresponding drug treatment. Mice in each group were subgrouped into 12 h , 1 d, 3 d and 5 d groups according to the time after treatment (n=6, each group). The neurological function was determined according to the reformed mNSS. The dry and wet weight method was used to the brain water content. RT-PCR technology was used to analysis the expressions of AQP-4 and MMP-9 mRNA in mice brain tissue. Results Each treatment group as compared with model group, mNSS was the highest (F=4.56, P<0.05). the brain water content was the lowest (F=5.38, P<0.05), the mRNA expressions of AQP-4 and MMP-9 was the least in combination Group(F=4.02, F=4.68, P<0.05) Conclusions Dexamethasone may relieve cerebral edema, improve the neural function in mice after brain hemorrhage and protect the brain tissue by regulating the transcriptional expression of AQP-4 and MMP-9. If combined with the high permeability dehydrant glycerin fructose, dexamethasone will further inhibit the progress of neurological impairment and brain edema. Key words: Fructors; Dexamethasone; Cerebral hemorrhage; Aquaporin 4; Matrix metalloproteinase 9