Effects of glutathione on expression and secretion of adipocytokines in high-fat diet rats to intermittent hypoxic

Abstract

Objective To investigate the effects of intermittent hypoxia on lipid metabolism, expressions and secretions of resistin and adiponectin in adipose tissue of high-fat diet rats, and the intervention of glutathione. Methods Forty-eight male Wistar rats were randomly divided into ordinary diet group and high-fat diet group, each group including control group, intermittent hypoxia group and intermittent hypoxic added glutathione treatment group.After four weeks, the serum levels of oxidative stress, blood lipid, resistin and adiponectin, and the mRNA and protein expressions of resistin and adiponectin in the adipose tissue were detected.The differences of the lipid metabolism, the expressions of resistin and adiponectin in adipose tissue, and the protective effects of glutathione were explored. Results After intermittent hypoxia exposure, resistin concentration in serum of rats was increased (P<0.05 or P<0.01), and adiponectin concentration in serum of rats was decreased (P<0.05 or P<0.01) .After glutathione treatment, the concentration of resistin was decreased (all P<0.05), and adiponect concentration was increased (all P<0.05). Resistin was negatively correlated with the level of oxidative stress, and positively correlated with the blood lipid abnormality.Adiponectin was the opposite.The mRNA and protein expressions of resistin and adiponectin in adipose tissue was in the same direction as their concentration changes in plasma. Conclusions Intermittent hypoxia could promote lipid metabolism disorder, abnormal expression and secretion of adipocytokines in the high-fat diet rats by oxidative stress, and glutathione has some protective effects. Key words: Intermittent hypoxia; High-fat diet; Lipid metabolism; Adipocytokines; Glutathione