Effects of global change on the ability of stream biofilm to dissipate the herbicide glyphosate

Abstract

The herbicide glyphosate is contaminating a large number of freshwater ecosystems worldwide and its fate and effects remains uncertain in light of the effects of global change. The present study examines how variations in water temperature and light availability relative to global change affect the ability of stream biofilms to degrade the herbicide glyphosate. Biofilms were exposed in microcosms to two levels of water temperature simulating global warming (Ambient = 19–22 °C and Warm = 21–24 °C) and three levels of light representative of riparian habitat destruction due to land use change (Dark = 0, Intermediate = 600, High = 1200 μmol photons m−2 s−1). Biofilms were acclimated to six different experimental treatments, namely i) ambient temperature without light (AMB_D), ii) ambient temperature and intermediate light (AMB_IL), iii) ambient temperature and high light (AMB_HL), iv) warm temperature without light (WARM_D), v) warm temperature and intermediate light (WARM_IL) and vi) warm temperature and high light (WARM_HL). The ability of biofilms to degrade 50 μg L−1 of glyphosate was tested. Results showed that water temperature increase, but not light availability increase, significantly increased aminomethyl phosphonic acid (AMPA) production by biofilms. However, the combined increase of temperature and light generated the shortest time to dissipate half of the glyphosate supplied and/or half of the maximum AMPA produced (6.4 and 5.4 days, respectively) by biofilms. Despite light had a major effect in modulating biofilm structural and functional descriptors, the response of certain descriptors (i. e. chlorophyll-a concentration, bacterial density and diversity, nutrient content and PHO activity) to light availability increase depended on water temperature. Specifically, the biofilms in the WARM_HL treatment displayed the highest Glucosidase: Peptidase and Glucosidase: Phosphatase enzyme activity ratios and the lowest biomass C: N molar ratios compared to the other treatments. According to these results, warmer temperatures and high light availability could have been exacerbating the decomposition of organic C compounds in biofilms, including the use of glyphosate as a C source for microbial heterotrophs. This study shows that ecoenzymatic stoichiometry and xenobiotic biodegradation approaches can be combined to better understand the functioning of biofilms in pesticide-polluted streams.