Abstract
Objective To explore the effects of Gingko biloba on immune function of rats exposed to arsenic, and to provide experimental evidence for prevention and treatment of arsenic poisoning. Methods By using a random number table, 40 Wistar rats were randomly divided into four groups according to body weight, 10 rats in each group, including the control, arsenic exposure, Ginkgo biloba treatment and natural recovery groups. The control group rats with normal diet ad libitum 90 d; other groups were freely given feed containing arsenic, 90 d; the natural recovery group rats were freely given arsenic(100 mg/kg) feed 90 d, and then given a normal diet for 45 d; Ginkgo biloba treatment rats ingested arsenic feed 90 d, and then give Ginkgo biloba solution(25 mg/kg · bw) orally, 6 d/week, treatment 45 d, fed normal normal diet during treatment. 24 h urine of rats were collected to measure the content of arsenic in urine. The blood of rats in heart were collected, to determine the positive rates of CD3+, CD4+, CD8+ cells and antibodies (IgG, IgM, IgA), complements (C3, C4). Results Urinary arsenic levels of rats in arsenic exposure, natural recovery, Ginkgo biloba treatment groups (2.991, 0.421, 0.334 mg/g) were higher than that of control group (0.141 mg/g,P 0.05). The positive rates of CD3+, CD4+ [(31.31±7.73)% , (25.94 ± 12.49)%] and CD4+/CD8+ ratio (0.91 ± 0.50) of arsenic exposure group in peripheral blood were lower than those of the control group [(60.83 ± 17.64)%, (42.11 ± 12.92)%, 1.80 ± 0.88, all P 0.05); the positive rates of CD3+, CD4+ cells [(54.00 ± 19.31)%, (39.93 ± 8.63)%] of Ginkgo biloba treatment group were significantly higher than those of the arsenic exposure group [(31.31 ± 7.73)%, (25.94 ± 12.49)%, all P 0.05]. Conclusions Arsenic can cause immune dysfunction in vivo in rat; even after ceased exposure to arsenic, the natural recovery is not good; Ginkgo biloba can enhance the immune function in arsenic poisoning rats. Key words: Ginkgo biloba; Arsenism; Rat; Immunity; Toxicity; Intervention
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