Abstract
Callus growth and plant regeneration from callus were studied for 6 species of Brassica differing in chromosome number and ploidy level and for 17 cultivars of B. oleracea having constant chromosome number and ploidy level. Callus was initiated from young hypocotyl explants and was maintained on media supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid (NAA), kinetin and coconut milk (CM). The rate of callus growth and the appearance of this tissue depended upon both the genotype examined and the growth medium. Variability in callus growth rate within B. oleracea was as great as that between the species of Brassica. Callus growth was less vigorous on a medium containing CM and high levels of 2,4-D than on a medium lacking CM and with a low concentration of 2,4-D; however, 2,4-D was shown to be necessary for callus formation. Organogenetic response of calluses tested on two regeneration media demonstrated intra-varietal variability as great as the variability between species. Rate of root and shoot organogesis was enhanced by a 1-week exposure to callus medium lacking 2,4-D.
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