Effects of genetic polymorphisms of GSTT1 and GSTM1 on metabolism of Polycyclic aromatic hydrocarbon in coal tar pitch workers

Abstract

Objective: To investigate the influence of genetic polymorphisms of GSTT1 and GSTM1 on metabolism of polycyclic aromatic hydrocarbon (PAHs) in coal tar pitch workers and to explore the effective bio-marker of occupational exposure to coal tar pitch. Methods: 374 cases of workers exposed to coal tar pitch work at the enterprise which use and product coal tar pitch in Shan Dong province named as exposure group.The ad-ministrative and the support staff 135 cases were selected as control group.The concentration of the metabolic product in urine by HPLC/MC.The genetic polymorphisms of GSTT1 and GSTM1 were detected by multiple PCR method. Results: The content of metabolic product in smoke group were significantly higher than workers do not smoke (P<0.05) . The content of metabolic product in exposure group were significantly higher than control group (P<0.05) . The content of metabolic product in workers carry positive GSTT1 and GSTM1 were significantly high-er than workers carry negative one (P<0.05) . The content of metabolic product in different types of labor were significantly different (P<0.05) . The content of metabolic product were significantly higher in urine after work than before work (P<0.05) . The content of metabolic product and workers carry positive GSTT1 and GSTM1 had a positive correlation (P<0.05) . Conclusion: Carrying positive GSTT1 and GSTM1 promote polycyclic aromatic hydrocarbons in the body's metabolism.