Effects of gender and temperature on oxidative stress enzymes in Nile tilapia Oreochromis niloticus exposed to paraquat

Abstract

Many classes of environmental pollutants can enhance the intracellular formation of reactive oxygen species, which can conduce to the damage of macromolecules and changes in oxidant defences levels in fish. In the present study it was analysed the hepatic levels of superoxide dismutase (SOD), glutathione reductase (GR), and glutathione S-transferase (GST) in males and females of Nile tilapia Oreochromis niloticus exposed to paraquat (PQ), at 17 and 27 °C. Tilapia were exposed to a sublethal concentration of PQ (0.5 mg L −1) during 45 days. Condition factor and hepatosomatic index of males and females exposed to PQ were significantly higher when compared with the control group, except in females at 17 °C. SOD and GST activities were higher in males and females exposed to PQ than in the control group at 17 and 27 °C. The levels of both enzyme activities revealed that they are sex-dependent with males exposed to PQ showing higher SOD activity (5.05 ± 0.13 and 4.84 ± 0.23 U/g protein, respectively at 17 and 27 °C) than females (4.21 ± 0.07 and 3.87 ± 0.27 U/g protein, respectively at the same temperature). Similar results were observed in GST activity. A GR activity significantly higher (9.09 ± 0.44 and 7.97 ± 1.08 U/g protein at 17 and 27 °C, respectively) was observed in PQ-exposed females, but not in exposed males. Fish exposed to PQ showed higher values of SOD and GST activities than the control group at both temperatures. These results are gender-dependent, while GR activity was higher only in PQ-exposed females. No significant differences were found for SOD, GST and GR activities between fish exposed to 17 and 27 °C, although males and females showed higher values at 17 °C. In short, this work advanced new knowledge on influence of gender in same biochemical parameters in tilapia exposed to PQ and demonstrated that their effects could be observed at different temperatures.