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Abstract
The aim of this study was evaluated the prevalence of Treg cells in peripheral blood in patients with gastric cancer, and investigate the effect of gastric cancer cells on their differentiation. ELISA was employed to assess the concentrations of TGF-β and IL-10 in gastric cancer patients' serum. Then, mouse gastric cancer cells were co-cultured with T lymphocytes or T lymphocytes + anti-TGF-β. Flow cytometric analysis and RT-PCR were then performed to detect Treg cells and TGF-β and IL-10 expression in gastric cancer cells. Our data showed that the expression of TGF-β and IL-10 in the patients with gastric cancer was increased compared to the case with healthy donors. The population of Treg cells and the expression levels of TGF-β and IL-10 in the co-culture group were much higher than in the control group (18.6% vs 9.5%) (P<0.05). Moreover, the population of Treg cells and the expression levels of TGF-β and IL-10 in the co-culture systerm were clearly decreased after addition of anti-TGF-β (7.7% vs 19.6%) (P<0.01). In conclusion, gastric cancer cells may induce Treg cell differentiation through TGF-β, and further promote immunosuppression.
Highlights
Gastric cancer is the fourth most common causes of cancer death in China (Thun et al, 2010)
Our data showed that the expression of TGF-β and IL-10 in the patients with gastric cancer was increased compared to the case with healthy donors
1B, the concentrations of TGF-β and IL-10 in Gastric cancer group were significantly higher than that in Normal group (P
Summary
Gastric cancer is the fourth most common causes of cancer death in China (Thun et al, 2010). It is well known that patients with gastric cancer have a poor immune response. A significantly increase of TGF-β expression has been described in gastric cancer cells (Achyut et al, 2011; Shen et al, 2012). Patients and experimental models with cancer showed that Treg cells down-regulated the activity of effector function against tumors, resulting in T-cell dysfunction in cancerbearing hosts (Weiss et al, 2012; Sakuishi et al, 2013). An increased population of Treg cells was reported in patients with ovarian cancer (Wicherek et al, 2011), lung cancer (Erfani et al, 2012), and breast cancer (Decker et al, 2012). There are no previous reports describing Treg cells in gastric cancer. Little is known about whether gastric cancer cells inducted the Treg cells through TGF-β
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