Effects of ganoderma lucidum polysaccharide peptide on phenotypic and functional maturation of dendritic cells from rat peripheral blood

Abstract

Objective To investigate the effects of ganoderma (GL-PP) lucidum polysaccharide peptide on the phenotype and function of rat dendritic cells (DC). Methods Monocytes isolated from rat peripheral blood were induced into DC by being cultured with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4. Flow cytometry, enzyme linked immunosorbent assay (ELISA) and mixed lymphocyte response were used to determine the effect of GL-PP on DC. Results The ratio of cell uptake fluoresceine isothiocyanate (FITC)- dextran (FITC-dextran) in GL-PP group [(35.33±4.62)%, (28.18±3.84)%, (26.52±2.01)%] was significantly lower than the control group [(58.42±3.84)%, P<0.01]; The expression of surface molecules major histocompatibility complex (MHC)-Ⅱ, CD40, CD80 and CD86[(92.6±1.42)%, (33.90±1.25)%, (89.97±1.19)%, (88.93±0.66)%] in GL-PP group (12.5 μg/ml) was significantly higher than the control group [(79.43±0.80)%, (5.47±0.21)%, (76.63±0.51)%, (68.63±0.78)%, P<0.01]. The allogeneic T cell-stimulatory capacity of DC in GL-PP group [(110.77±3.53)%, (112.14±4.33)%, (110.77±3.56)%] was enhanced compare with the control group [(100.00±1.78)%, P<0.01]. In addition, GL-PP could promote secretion of interleukin (IL)-12p70 and IL-10 from DC. Conclusion These data suggest that GL-PP promotes the phenotypic and functional maturation of DCs. Key words: Ganoderma lucidum polysaccharide; Dendritic cells; Immunomodulatory