Effects of gamma radiation treatment on three different medicinal plants: Microbial limit test, total phenolic content, in vitro cytotoxicity effect and antioxidant assay

Abstract

The aim of this study is to evaluate the effects of gamma radiation treatment on three medicinal plants, namely Euodia malayana, Gnetum gnemon and Khaya senegalensis at two different forms; methanol leaf extracts and dried leaves respectively. The microbial limit test (MLT) studies indicated the suitable dosage of minimum and maximum gamma irradiation for leaf extracts as well as dried leaves of all the tested medicinal plants. Quantitative analysis of total phenolic content (TPC) analysis is based on calorimetric measurements determined using the Folin-Ciocalteu reagent with gallic acid (GA) used as the reference. In vitro cytotoxicity assay by using fibroblast (L929) cell lines was performed on each plant to determine the toxicity effect which sodium dodecyl sulfate (SDS) as the positive control. DPPH (2,2-diphenyl-1-picryl-hydrazyl) assay was conducted by using vitamin C and GA as the positive controls to determine the antioxidant property of each plant. The MLT analysis indicated that the suitable dosage gamma irradiation for leaf extracts was 6-12kGy and dried leaves were 9-13kGy. The amount of GA concentration in each plant increased significantly from 30-51mg GAE g-1 before treatment to 57-103mg GAE g-1 after treatment with gamma radiation. This showed no significant effect of in vitro cytotoxicity activity before and after treatment with gamma irradiation in this study. Effective concentration (EC50) values of Khaya senegalensis plant reduced significantly (P≤0.005) from 44.510μg/ml before treatment to 24.691μg/ml after treatment with gamma radiation, which indicate an increase of free radical scavenging activity.